Akhter, S., Vignini, A., Wen, Z., English, Ann M. 
ORCID: https://orcid.org/0000-0002-3696-7710, Wang, P. G. and Mutus, B.
(2002)
Evidence for S-nitrosothiol-dependent changes in fibrinogen that do not involve transnitrosation or thiolation.
Proceedings of the National Academy of Sciences, 99
(14).
pp. 9172-9177.
ISSN 0027-8424
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Official URL: https://doi.org/10.1073/pnas.142136499
Abstract
S-nitrosoglutathione (GSNO, 50 μM) inhibited the initial rate of thrombin-catalyzed human and bovine fibrinogen polymerization by ≈50% to 68% respectively. Inhibition was also observed with other structurally varied S-nitrosothiols (RSNOs) including sugar derivatives of S-nitroso-N-acetylpenicillamine (SNAP). The fact that the same concentration of GSNO had no effect on thrombin-dependent hydrolysis of tosylglycylprolylarginine-4-nitroanilide acetate suggested that this inhibition was due to GSNO-induced changes in fibrinogen structure. This result was confirmed by CD spectroscopy where GSNO or S-nitrosohomocysteine increased the α-helical content of fibrinogen by ≈15% and 11%, respectively. S-carboxymethylamido derivatives of glutathione or homocysteine had no effect on the fibrinogen secondary structure. The GSNO-dependent secondary structural effects were reversed on gel filtration chromatography, suggesting that the effects were allosteric. Further evidence for fibrinogen–GSNO interactions was obtained from GSNO-dependent quenching of the intrinsic fibrinogen Trp fluorescence and the perturbation of the GSNO circular dichroic absorbance as a function of [fibrinogen]. The Kds of 3 to 10 μM for fibrinogen–GSNO interactions with a stoichiometry of 2:1 (GSNO:fibrinogen) were estimated from isothermal titration calorimetry and fluorescence quenching, respectively. These results suggest that RSNOs induce changes to fibrinogen structure by interacting at specific aromatic rich domains. Three such putative RSNO-binding domains have been identified in the unordered, aromatic residue-rich C-termini of the α-chains of fibrinogen.
| Divisions: | Concordia University > Faculty of Arts and Science > Chemistry and Biochemistry |
|---|---|
| Item Type: | Article |
| Refereed: | Yes |
| Authors: | Akhter, S. and Vignini, A. and Wen, Z. and English, Ann M. and Wang, P. G. and Mutus, B. |
| Journal or Publication: | Proceedings of the National Academy of Sciences |
| Date: | 9 July 2002 |
| Digital Object Identifier (DOI): | 10.1073/pnas.142136499 |
| ID Code: | 985174 |
| Deposited By: | MIA MASSICOTTE |
| Deposited On: | 28 May 2019 14:11 |
| Last Modified: | 29 May 2019 12:43 |
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