Cheung, E., Taylor, K., Kornblatt, Jack A. 
ORCID: https://orcid.org/0000-0002-9802-8321, English, Ann M. ORCID: https://orcid.org/0000-0002-3696-7710, McLendon, G. and Miller, J. R.
(1986)
Direct measurements of intramolecular electron transfer rates between cytochrome c and cytochrome c peroxidase: effects of exothermicity and primary sequence on rate.
Proceedings of the National Academy of Sciences, 83
(5).
pp. 1330-1333.
ISSN 0027-8424
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Official URL: https://doi.org/10.1073/pnas.83.5.1330
Abstract
Rapid mixing of ferrocytochrome c peroxidase [cyt c peroxidase(II)] and ferricytochrome c [cyt c(III)] results in the reduction of cyt c(III) by cyt c peroxidase(II). In 10 mM phosphate, pH 7.0, the rate of decay of cyt c peroxidase(II) and the rate of accumulation of cyt c(II) give equal first-order rate constants: k = 0.23 +/- 0.02 s-1. Equivalent results are obtained by pulse radiolysis using isopropanol radical as the reducing agent. This rate is independent of the initial cyt c(III):cyt c peroxidase(II) ratios. These results are consistent with unimolecular electron transfer occurring within a cyt c(III)-cyt c peroxidase(II) complex. When cyt c is replaced by porphyrin cyt c (iron-free cyt c), a complex still forms with cyt c peroxidase. On radiolysis, using e-aq as the reducing agent, intracomplex electron transfer occurs from the porphyrin cyt c anion radical to cyt c peroxidase(III) with k = 150 s-1. This large rate increase with increasing delta G degrees suggests that the barrier for intracomplex electron transfer is large. Finally, we have briefly investigated how the cyt c peroxidase(II)----cyt c(III) rate depends on the primary structure of cyt c(III). We find the reactivity order to be as follows: yeast (k = 3.4 s-1) greater than horse (k = 0.3 s-1) greater than tuna (k = 0.2 s-1). These results mirror a report [Ho, P. S., Sutoris, C., Liang, N., Margoliash, E. & Hoffman, B. M. (1985) J. Am. Chem. Soc. 107, 1070-1071] on excited state reactions of the cyt c/cyt c peroxidase couple.
| Divisions: | Concordia University > Faculty of Arts and Science > Chemistry and Biochemistry |
|---|---|
| Item Type: | Article |
| Refereed: | Yes |
| Authors: | Cheung, E. and Taylor, K. and Kornblatt, Jack A. and English, Ann M. and McLendon, G. and Miller, J. R. |
| Journal or Publication: | Proceedings of the National Academy of Sciences |
| Date: | March 1986 |
| Digital Object Identifier (DOI): | 10.1073/pnas.83.5.1330 |
| ID Code: | 985185 |
| Deposited By: | MIA MASSICOTTE |
| Deposited On: | 28 May 2019 14:00 |
| Last Modified: | 29 May 2019 13:35 |
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