Login | Register

Cue-alcohol associative learning in female rats


Cue-alcohol associative learning in female rats

Chaudhri, Nadia ORCID: https://orcid.org/0000-0003-4217-4044, Cofresí, Roberto U., Monfils, Marie-H., Gonzales, Rueben A. and Lee, Hongjoo J. (2019) Cue-alcohol associative learning in female rats. Alcohol . ISSN 07418329 (In Press)

[thumbnail of Chaudhri 2019.pdf]
Text (application/pdf)
Chaudhri 2019.pdf - Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Official URL: http://dx.doi.org/10.1016/j.alcohol.2019.03.003


The ability of environmental cues to trigger alcohol seeking behaviors is believed to facilitate problematic alcohol use. We previously showed that the development of this cue-evoked alcohol approach reflects cue-alcohol learning and memory in the adult male rat; however, we do not know whether the same is true for similarly aged female rats. Consequently, adult Long-Evans female rats were allowed to drink unsweetened alcohol in the homecage (MWF 24 hr two-bottle choice, 5 weeks) and subsequently split into two experimental groups: paired and unpaired. Groups were matched for ingested doses and alcohol bottle preference across the pre-conditioning homecage period. Both groups were trained in conditioning chambers using a Pavlovian procedure. For the paired group, the chamber houselight was illuminated to signal access to an alcohol sipper. Houselight onset was yoked for the unpaired group, but access to the alcohol sipper was scheduled to occur only during the intervening periods (in the absence of light). We found that in the paired, but not unpaired group, an alcohol approach reaction was conditioned to houselight illumination, and the level of cue-conditioned reactivity predicted drinking behavior within trials. Groups experienced equivalently low but non-negligible blood alcohol concentrations over the course of conditioning sessions. We conclude that cue-triggered alcohol seeking behavior in adult female rats reflects associative learning about the relationship between alcohol availability and houselight illumination.

Divisions:Concordia University > Faculty of Arts and Science > Psychology
Item Type:Article
Authors:Chaudhri, Nadia and Cofresí, Roberto U. and Monfils, Marie-H. and Gonzales, Rueben A. and Lee, Hongjoo J.
Journal or Publication:Alcohol
  • Canadian Institutes of Health Research (CIHR)
Digital Object Identifier (DOI):10.1016/j.alcohol.2019.03.003
Keywords:oral alcohol; low dose; Pavlovian conditioning; female rat; cue reactivity
ID Code:985531
Deposited By: ALINE SOREL
Deposited On:25 Jun 2019 20:21
Last Modified:19 Mar 2020 00:00


L.C. Anderson, G.D. Petrovich. Renewal of conditioned responding to food cues in rats: Sex differences and relevance of estradiol, Physiology and Behavior, 151 (2015), pp. 338-344 https://doi.org/10.1016/j.physbeh.2015.07.035

J.M. Barker, J.R. Taylor. Sex differences in incentive motivation and the relationship to the development and maintenance of alcohol use disorders, Physiology and Behavior (May) (2017), pp. 0-1 https://doi.org/10.1016/j.physbeh.2017.09.027

J.B. Becker, G.F. Koob. Sex Differences in Animal Models: Focus on Addiction, Pharmacological Reviews (68) (2016), pp. 242-263

M.L. Bertholomey, V. Nagarajan, M.M. Torregrossa. Sex differences in reinstatement of alcohol seeking in response to cues and yohimbine in rats with and without a history of adolescent corticosterone exposure, Psychopharmacology, 233 (12) (2016), pp. 2277-2287 https://doi.org/10.1007/s00213-016-4278-x

S.A.M. Bobzean, T.S. Dennis, L.I. Perrotti. Acute estradiol treatment affects the expression of cocaine-induced conditioned place preference in ovariectomized female rats, Brain Research Bulletin, 103 (2014), pp. 49-53 https://doi.org/10.1016/j.brainresbull.2014.02.002

J.O. Campbell, R.D. Wood, L.P. Spear. Cocaine and morphine-induced place conditioning in adolescent and adult rats, Physiology and Behavior, 68 (4) (2000), pp. 487-493 https://doi.org/10.1016/S0031-9384(99)00225-5

J. Carrillo, E.C. Howard, M. Moten, B.D. Houck, C.L. Czachowski, R.A. Gonzales. A 3-day exposure to 10% ethanol with 10% sucrose successfully initiates ethanol self-administration, Alcohol, 42 (3) (2008), pp. 171-178 https://doi.org/10.1016/j.alcohol.2008.01.005

R.U. Cofresí, D.J. Grote, E.V.T. Le, M.-H. Monfils, N. Chaudhri, R.A. Gonzales, H.J. Lee. Alcohol-associated antecedent stimuli elicit alcohol seeking in non-dependent rats and may activate the insula, Alcohol, 76 (2019), pp. 91-102 https://doi.org/10.1016/j.alcohol.2018.08.004

R.U. Cofresí, H.J. Lee, M.-H. Monfils, N. Chaudhri, R.A. Gonzales. Characterizing conditioned reactivity to sequential alcohol-predictive cues in well-trained rats, Alcohol, 69 (2018), pp. 41-49 https://doi.org/10.1016/j.alcohol.2017.11.034

R.U. Cofresí, S.M. Lewis, N. Chaudhri, H.J. Lee, M.-H. Monfils, R.A. Gonzales. Postretrieval Extinction Attenuates Alcohol Cue Reactivity in Rats, Alcoholism: Clinical and Experimental Research, 41 (3) (2017), pp. 608-617 https://doi.org/10.1111/acer.13323

A. Erol, A.M.-C. Ho, S.J. Winham, V.M. Karpyak. Sex hormones in alcohol consumption: a systematic review of evidence, Addiction Biology (2017), pp. 157-169 https://doi.org/10.1111/adb.12589

M.W. Feltenstein, A.R. Henderson, R.E. See. Enhancement of cue-induced reinstatement of cocaine-seeking in rats by yohimbine: Sex differences and the role of the estrous cycle, Psychopharmacology, 216 (1) (2011), pp. 53-62 https://doi.org/10.1007/s00213-011-2187-6

E.R. Hilderbrand, A.W. Lasek. Estradiol enhances ethanol reward in female mice through activation of ERα and ERβ, Hormones and Behavior, 98 (January) (2018), pp. 159-164 https://doi.org/10.1016/j.yhbeh.2018.01.001

P.C. Holland, R.A. Rescorla. The effect of two ways of devaluing the unconditioned stimulus after first- and second-order appetitive conditioning, Journal of Experimental Psychology: Animal Behavior Processes, 1 (4) (1975), pp. 355-363 https://doi.org/10.1037//0097-7403.1.4.355

T.E. Kippin, R.A. Fuchs, R.H. Mehta, J.M. Case, M.P. Parker, H.A. Bimonte-Nelson, R.E. See. Potentiation of cocaine-primed reinstatement of drug seeking in female rats during estrus, Psychopharmacology, 182 (2) (2005), pp. 245-252 https://doi.org/10.1007/s00213-005-0071-y

C.P. Knight, S.R. Hauser, G.A. Deehan, J.E. Toalston, W.J. Mcbride, Z.A. Rodd. Oral Conditioned Cues Can Enhance or Inhibit Ethanol (EtOH)-Seeking and EtOH-Relapse Drinking by Alcohol-Preferring (P) Rats, Alcoholism: Clinical & Experimental Research, 40 (4) (2016), pp. 906-915 https://doi.org/10.1111/acer.13027

T.A. Kosten, H.J. Lee, J.J. Kim. Early life stress impairs fear conditioning in adult male and female rats, Brain Research, 1087 (1) (2006), pp. 142-15 https://doi.org/10.1016/j.brainres.2006.03.009

M.D. Krank. Pavlovian conditioning with ethanol: sign-tracking (autoshaping), conditioned incentive, and ethanol self-administration, Alcoholism: Clinical & Experimental Research, 27 (10) (2003), pp. 1592-1598 https://doi.org/10.1097/01.ALC.0000092060.09228.DE

M.D. Krank, S. O’Neill, K. Squarey, J. Jacob. Goal- and signal-directed incentive: conditioned approach, seeking, and consumption established with unsweetened alcohol in rats, Psychopharmacology, 196 (3) (2008), pp. 397-405 https://doi.org/10.1007/s00213-007-0971-0

R.J. Lamb, B.C. Ginsburg, A. Greig, C.W. Schindler. Effects of Rat Strain and Method of Inducing Ethanol Drinking on Pavlovian-Instrumental-Transfer with Ethanol-Paired Conditioned Stimuli, Alcohol (2019) https://doi.org/10.1016/j.alcohol.2019.01.003

R.J. Lamb, B.C. Ginsburg, C.W. Schindler. Effects of an ethanol-paired CS on responding for ethanol and food: Comparisons with a stimulus in a Truly-Random-Control group and to a food-paired CS on responding for food, Alcohol, 57 (2016), pp. 15-27 https://doi.org/10.1016/j.alcohol.2016.10.009

M.R. LeCocq, S. Lahlou, M. Chahine, L.N. Padillo, N. Chaudhri. Modeling Relapse to Pavlovian Alcohol-Seeking in Rats Using Reinstatement and Spontaneous Recovery Paradigms, Alcoholism: Clinical and Experimental Research, 42 (9) (2018), pp. 1795-1806 https://doi.org/10.1111/acer.13825

H.J. Lee, F. Groshek, G.D. Petrovich, J.P. Cantalini, M. Gallagher, P.C. Holland. Role of amygdalo-nigral circuitry in conditioning of a visual stimulus paired with food, The Journal of Neuroscience : The Official Journal of the Society for Neuroscience, 25 (15) (2005), pp. 3881-3888, https://doi.org/10.1523/JNEUROSCI.0416-05.2005

M.R. Milad, S.A. Igoe, K. Lebron-Milad, J.E. Novales. Estrous cycle phase and gonadal hormones influence conditioned fear extinction, Neuroscience, 164 (3) (2009), pp. 887-895 https://doi.org/10.1016/j.neuroscience.2009.09.011

E.Z. Millan, R.M. Reese, C.D. Grossman, N. Chaudhri, P.H. Janak. Nucleus Accumbens and Posterior Amygdala Mediate Cue-Triggered Alcohol Seeking and Suppress Behavior During the Omission of Alcohol-Predictive Cues, Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology, 40 (11) (2015), pp. 2555-2565 https://doi.org/10.1038/npp.2015.102

S.E. Morrison, M.A. Bamkole, S.M. Nicola. Sign tracking, but not goal tracking, is resistant to outcome devaluation, Frontiers in Neuroscience, 9 (DEC) (2015), pp. 1-12 https://doi.org/10.3389/fnins.2015.00468

T.B. Nentwig, K.P. Myers, J.E. Grisel. Initial subjective reward to alcohol in Sprague-Dawley rats, Alcohol, 58 (2017), pp. 19-22 https://doi.org/10.1016/j.alcohol.2016.11.005

K.K. Pitchers, S.B. Flagel, E.G. O’Donnell, L.C. Solberg Woods, M. Sarter, T.E. Robinson. Individual variation in the propensity to attribute incentive salience to a food cue: Influence of sex, Behavioural Brain Research, 278 (0) (2015), pp. 462-469 https://doi.org/10.1016/j.bbr.2014.10.036

B.M. Priddy, S.A. Carmack, L.C. Thomas, J.C.M. Vendruscolo, G.F. Koob, L.F. Vendruscolo. Sex, strain, and estrous cycle influences on alcohol drinking in rats, Pharmacology Biochemistry and Behavior, 152 (2017), pp. 61-67 https://doi.org/10.1016/j.pbb.2016.08.001

C.R. Pryce, J. Lehmann, J. Feldon. Effect of sex on fear conditioning is similar for context and discrete CS in Wistar, Lewis and Fischer rat strains, Pharmacology Biochemistry and Behavior, 64 (4) (1999), pp. 753-759, https://doi.org/10.1016/S0091-3057(99)00147-1

P.A. Randall, R.T. Stewart, J. Besheer. Sex differences in alcohol self-administration and relapse-like behavior in Long-Evans rats, Pharmacology Biochemistry and Behavior, 156 (March) (2017), pp. 1-9 https://doi.org/10.1016/j.pbb.2017.03.005

A.J. Roberts, A.D. Smith, F. Weiss, C. Rivier, G.F. Koob. Estrous Cycle Effects on Operant Responding for Ethanol in Female Rats, Alcoholism: Clinical & Experimental Research, 22 (7) (1998), pp. 1564-1569 https://doi.org/10.1111/j.1530-0277.1998.tb03950.x

S.J. Russo, E.D. Festa, S.J. Fabian, F.M. Gazi, M. Kraish, S. Jenab, V. Quiñones-Jenab. Gonadal hormones differentially modulate cocaine-induced conditioned place preference in male and female rats, Neuroscience, 120 (2) (2003), pp. 523-533 https://doi.org/10.1016/S0306-4522(03)00317-8

S.J. Russo, S. Jenab, S.J. Fabian, E.D. Festa, L.M. Kemen, V. Quinones-Jenab. Sex differences in the conditioned rewarding effects of cocaine, Brain Research, 970 (1–2) (2003), pp. 214-220 https://doi.org/10.1016/S0006-8993(03)02346-1

H.H. Samson, C.L. Czachowski, C.J. Slawecki. A New Assessment of the Ability of Oral Ethanol to Function as a Reinforcing Stimulus, Alcoholism: Clinical & Experimental Research, 24 (6) (2000), pp. 766-773 https://doi.org/10.1111/j.1530-0277.2000.tb02054.x

H.H. Samson, C.J. Slawecki, A.L. Sharpe, A. Chappell. Appetitive and consummatory behaviors in the control of ethanol consumption: a measure of ethanol seeking behavior, Alcoholism: Clinical & Experimental Research, 22 (8) (1998), pp. 1783-1787

J. Sharp, T. Zammit, T. Azar, D. Lawson. Stress-like responses to common procedures in individually and group-housed female rats, Contemporary Topics in Laboratory Animal Science/American Association for Laboratory Animal Science, 42 (1) (2003), pp. 9-18

L.K. Sherrill, C. Berthold, W.A. Koss, J.M. Juraska, J.M. Gulley. Sex differences in the effects of ethanol pre-exposure during adolescence on ethanol-induced conditioned taste aversion in adult rats, Behavioural Brain Research, 225 (1) (2011), pp. 104-109 https://doi.org/10.1016/j.bbr.2011.07.003

L.M. Sparks, J.M. Sciascia, Z. Ayorech, N. Chaudhri. Vendor differences in alcohol consumption and the contribution of dopamine receptors to Pavlovian-conditioned alcohol-seeking in Long-Evans rats, Psychopharmacology, 231 (4) (2014), pp. 753-764 https://doi.org/10.1007/s00213-013-3292-5

C.S. Srey, J.-M.N. Maddux, N. Chaudhri. The attribution of incentive salience to Pavlovian alcohol cues: a shift from goal-tracking to sign-tracking, Frontiers in Behavioral Neuroscience, 9 (March) (2015), p. 54 https://doi.org/10.3389/fnbeh.2015.00054

A. Tomie. Locating Reward Cue at Response Manipulandum (CAM) Induces Symptoms of Drug Abuse, Neuroscience & Biobehavioral Reviews, 20 (3) (1996), pp. 505-535 https://doi.org/10.1016/0149-7634(95)00023-2

A. Tomie, E.D. Festa, D.R. Sparta, L.A. Pohorecky. Lever conditioned stimulus-directed autoshaping induced by saccharin-ethanol unconditioned stimulus solution: Effects of ethanol concentration and trial spacing, Alcohol, 30 (1) (2003), pp. 35-44 https://doi.org/10.1016/S0741-8329(03)00069-7

A. Tomie, T. Kuo, K.R. Apor, K.E. Salomon, L.A. Pohorecky. Autoshaping induces ethanol drinking in nondeprived rats: Evidence of long-term retention but no induction of ethanol preference, Pharmacology Biochemistry and Behavior, 77 (4) (2004), pp. 797-804 https://doi.org/10.1016/j.pbb.2004.02.005

A. Tomie, W.C. Miller, E. Dranoff, L.A. Pohorecky. Intermittent presentations of ethanol sipper tube induce ethanol drinking in rats, Alcohol and Alcoholism (Oxford, Oxfordshire), 41 (3) (2006), pp. 225-230 https://doi.org/10.1093/alcalc/agl002

A. Tomie, N. Sharma. Pavlovian Sign-Tracking Model of Alcohol Abuse, Current Drug Abuse Reviews, 6 (2) (2013), pp. 1-19

O.V. Torres, E.M. Walker, B.S. Beas, L.E. O’Dell. Female rats display enhanced rewarding effects of ethanol that are hormone dependent, Alcoholism: Clinical & Experimental Research, 38 (1) (2014), pp. 108-115 https://doi.org/10.1111/acer.12213

F.R. Villaruel, N. Chaudhri. Individual Differences in the Attribution of Incentive Salience to a Pavlovian Alcohol Cue, Frontiers in Behavioral Neuroscience, 10 (December) (2016), pp. 1-13 https://doi.org/10.3389/fnbeh.2016.00238
All items in Spectrum are protected by copyright, with all rights reserved. The use of items is governed by Spectrum's terms of access.

Repository Staff Only: item control page

Downloads per month over past year

Research related to the current document (at the CORE website)
- Research related to the current document (at the CORE website)
Back to top Back to top