Valdez López, Dulce María (2023) Identification of Glycosyltransferase Inhibitor Molecules Targeting Cell-Surface Fucosylation Using High-Throughput Methods. Masters thesis, Concordia University.
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Abstract
In cancers, increased fucosylation of cell surface glycans is a trademark of malignant cell transformation and contributes to many aberrant cellular events during cancer development. Alterations in the expression of fucosylated oligosaccharides is mainly attributed to the upregulation of specific fucosyltransferase enzymes (FUTs), which construct fucosylated glycans using GDP-fucose as a substrate. FUT5 and FUT6 are two important enzymes of the a1-3-fucosyltransferase family that act to form sialyl LewisX, a member of the Lewis antigen family that is abnormally abundant in numerous cancers and is associated with advanced tumor grade poor prognosis due to its capacity to interact with E- and L- selectin molecules expressed by the endothelium, thus aiding in metastasis by direct binding of cancer cells.
To identify inhibitors of FUT5 and FUT6 enzymes as potential cancer therapeutics, we have developed a high-throughput coupled enzyme assay that makes use of 3’-sialyl-N-acetyllactosamine-resorufin (3’-SLN-Res), a fluorogenically labeled sialyl LewisX precursor. When non-fucosylated, this synthetic substrate is recognized and hydrolyzed by specific glycoside hydrolase enzymes (glycosidases) to release fluorescent resorufin, yet if the probe is fucosylated by FUT5 or FUT6 prior to treatment with glycosidases, these cannot act on the substrate and no fluorescent signal is produced. Employing this assay, we were able to screen a library of bioactive small-molecule compounds in a fully automated high-throughput screening and identify several hits, demonstrating that our approach can be used to single out potent FUT inhibitors from commercial compound libraries in a microtiter plate format.
Divisions: | Concordia University > Faculty of Arts and Science > Biology |
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Item Type: | Thesis (Masters) |
Authors: | Valdez López, Dulce María |
Institution: | Concordia University |
Degree Name: | M. Sc. |
Program: | Biology |
Date: | 1 August 2023 |
Thesis Supervisor(s): | Kwan, David H. |
ID Code: | 993153 |
Deposited By: | Dulce Valdez Lopez |
Deposited On: | 04 Jun 2024 14:29 |
Last Modified: | 04 Jun 2024 14:29 |
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