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The Effects of Exogenous Cardiolipin on Skeletal Muscle, Aerobic Exercise and Anxiety-Related Behaviour

Title:

The Effects of Exogenous Cardiolipin on Skeletal Muscle, Aerobic Exercise and Anxiety-Related Behaviour

Laplante, Amanda (2024) The Effects of Exogenous Cardiolipin on Skeletal Muscle, Aerobic Exercise and Anxiety-Related Behaviour. Masters thesis, Concordia University.

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Abstract

Cardiolipin is a phospholipid found in the inner mitochondrial membrane and, consequently, is involved in energy metabolism. Cardiolipin is released from necrotic cells following myocardial
ischemia and reperfusion injury. Furthermore, increased levels of anxiety have been seen in individuals following myocardial infarction. Despite its various effects on liver, smooth and
cardiac muscle, the impact of increased cardiolipin on skeletal muscle and anxiety-like behaviour remains unknown. PURPOSE. This two-part project investigated the effects of exogenous cardiolipin on voluntary exercise metrics, forced exercise training, functional aerobic capacity, anxiety-related behaviors, and mitochondrial respiration of skeletal muscle in a mouse
model. METHODS. C57BL/6 mice were randomized to an experimental and a control group. The mice were injected 2x/week with 0.1ml of cardiolipin (0.25mg/ml or 0.5mg/ml) or placebo
solution. In-cage running wheels measured voluntary running, while high-intensity interval training was used as a method of exercise training. Aerobic capacity was assessed by maximal
endurance or maximal graded exercise tests. Open-field test and elevated plus maze served to measure anxiety-related behaviours. Lastly, mitochondrial respiratory capacity of the vastus
lateralis muscle was measured by high-resolution respirometry. SIGNIFICANCE. Overall, this
research has significant implications for advancing our understanding of cardiolipin biology,
cardiovascular physiology, skeletal muscle function, and mental health, with potential
implications for developing novel therapeutic interventions and improving patient outcomes.

Divisions:Concordia University > Faculty of Arts and Science > Exercise Science
Item Type:Thesis (Masters)
Authors:Laplante, Amanda
Institution:Concordia University
Degree Name:M.A. Sc.
Program:Health and Exercise Science
Date:18 July 2024
Thesis Supervisor(s):Bergdahl, Andreas
Keywords:Cardiolipin; vastus lateralis; running capacity; voluntary exercise; oxidative phosphorylation
ID Code:994235
Deposited By: Amanda Laplante
Deposited On:24 Oct 2024 17:22
Last Modified:24 Oct 2024 17:22

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