Velosa, Steffy, Turner, Sarah E. and Jaeger, Jochen A.G. (2025) Spatial distribution of wildlife road mortality: How important is rigorous data collection? Transportation Research Part D: Transport and Environment, 149 (105016). pp. 1-21.
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Official URL: https://doi.org/10.1016/j.trd.2025.105016
Abstract
We conducted 66 road mortality surveys along a 31.5 km stretch of a high-traffic 4-lane highway between Montreal and Sherbrooke in Québec, Canada. Surveys by vehicle between May and August 2019 recorded 212 animal carcasses from 48 species. Hotspots and coldspots for ground-dwelling vertebrates (mammals, amphibians, and reptiles) and birds were identified at four scales. The number of hotspots was higher at finer scales, while the combined length of hotspots was greater at coarser scales. We found significantly more animals and identified more species than the highway patrol, suggesting that rigorous road mortality surveys are beneficial for better understanding road impacts on biodiversity. We estimated that the highway patrol’s reporting probability for medium-sized mammals (more than 0.65 kg, less than 30 kg) was between 21 % and 54 % that of our systematic surveys. We recommend priority locations for mitigation to reduce road mortality and re-establish connectivity between wildlife populations separated by the highway.
| Divisions: | Concordia University > Faculty of Arts and Science > Geography, Planning and Environment |
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| Item Type: | Article |
| Refereed: | Yes |
| Authors: | Velosa, Steffy and Turner, Sarah E. and Jaeger, Jochen A.G. |
| Journal or Publication: | Transportation Research Part D: Transport and Environment |
| Date: | 1 December 2025 |
| Projects: |
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| Funders: |
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| Digital Object Identifier (DOI): | 10.1016/j.trd.2025.105016 |
| Keywords: | Connectivity, Data collection, Fences, Hotspots, Mitigation, Mortality surveys, Road ecology, Roadkill, Wildlife-vehicle collisions (WVCs). |
| ID Code: | 996397 |
| Deposited By: | Jochen Jaeger |
| Deposited On: | 28 Oct 2025 15:52 |
| Last Modified: | 28 Oct 2025 15:52 |
References:
Anderson, M.G., Vickery, B., Gorman, M., Gratton, L., Morrison, M., Maillet, J., Olivero, A., Ferree, C., Morse, D., Kehm, G., Rosalska, K., Khanna, S., Bernstein, S., 2006. The Northern Appalachian/Acadian Ecoregion: Conservation Assessment Status and Trends: 2006. The Nature Conservancy Eastern Regional Science in collaboration with The Nature Conservancy of Canada: Atlantic and Quebec Regions. Boston, MA. Online: http://support.natureconservancy.ca/pdf/blueprints/Northern_Appalachians_Acadia.pdf.Ascens˜ao, F., Clevenger, A., Santos-Reis, M., Urbano, P., Jackson, N., 2013. Wildlife-vehicle collision mitigation: Is partial fencing the answer? An agent-based model approach. Ecological Modelling 257, 36–43. https://doi.org/10.1016/j.ecolmodel.2013.02.026.
Balciauskas, L., Stratford, J., Balciauskiene, L., Kucas, A., 2020. Importance of professional roadkill data in assessing diversity of mammal roadkills. Transport. Res. Part D: Trans. Environ. 87, 102493. https://doi.org/10.1016/j.trd.2020.102493.
Barrett, H., Vanlaar, W.G.M., Robertson, R.D., 2023. Wildlife-Vehicle Collisions in Canada 2000-2020. Fact sheet. Online: https://tirf.ca/news/wildlife-vehicle-fatal-crash-data-guide-prevention-strategies/.
Bédard, Y., Trottier, D., 2009. Prolongement de l’autoroute Robert-Bourassa en boulevard urbain à Québec. Un défi d’intégration environnementale. Dossier de candidature au prix de réalisation environnementale 2008 soumis à l’Association des transports du Canada, ministère des Transports du Québec. 11 pp. Online: https://www.tac-atc.ca/wp-content/uploads/mtq-bourassa-f.pdf.
Bouffard, M., Leblanc, Y., Bédard, Y., Martel, D., 2012. Impacts de clôtures métalliques et de passages fauniques sur la sécurité routière et le déplacement des orignaux le long de la route 175 au Québec. Le Nat. Can. 136 (2), 8–15. https://doi.org/10.7202/1009100ar.
Brunen, B., Daguet, C., Jaeger, J.A.G., 2020. What attributes are relevant for drainage culverts to serve as efficient road crossing structures for mammals? J. Environ. Managem. 268, 110423. https://doi.org/10.1016/j.jenvman.2020.110423.
Cerqueira, R.C., Leonard, P., Gonçalves da Silva, L., Bager, A., Clevenger, A.P., Jaeger, J.A.G., Grilo, C., 2021. Potential movement corridors and high road-kill likelihood do not spatially coincide for felids in Brazil: Implications for road mitigation. Environ. Managem. 57, 412–423. https://doi.org/10.1007/s00267-020-01411-4.
Clevenger, A.P., Chruszcz, B., Gunson, K.E., 2003. Spatial patterns and factors influencing small vertebrate fauna road-kill aggregations. Biol. Conserv. 109 (1), 15–26. https://doi.org/10.1016/S0006-3207(02)00127-1.
Coelho, I.P., Teixeira, F.Z., Colombo, P., Coelho, A.V.P., Kindel, A., 2012. Anuran road-kills neighboring a peri-urban reserve in the Atlantic Forest. Brazil. J. Environ. Managem. 112, 17–26. https://doi.org/10.1016/j.jenvman.2012.07.004.
Coelho, A.V.P., Coelho, I.P., Teixeira, F.Z., Kindel, A., 2014. Siriema: Road Mortality Software. NERF/UFRGS, Porto Alegre, Brazil. www.ufrgs.br/siriema (accessed March 2019).
Corridor appalachien, 2019a. Fiches Techniques: Biodiversité. Retrieved from: http://www.corridorappalachien.ca/biodiversite/.
Corridor appalachien, 2019b. Identification des grands mammifères. Animal Identification Index.
Corridor appalachien, 2020. Couche numérique du réseau écologique identifié sur le territoire d’action de Corridor appalachien. Eastman: Corridor appalachien.
COSEWIC, 2008. COSEWIC assessment and status report on the Snapping Turtle Chelydra serpentina in Canada. Committee on the Status of Endangered Wildlife in Canada. Ottawa. vii +47 pp.
COSEWIC, 2018. COSEWIC assessment and status report on the Midland Painted Turtle Chrysemys picta marginata and the Eastern Painted Turtle Chrysemys picta picta in Canada. Committee on the Status of Endangered Wildlife in Canada. Ottawa. xvi +107 pp. (http://www.registrelepsararegistry.gc.ca/default.asp?lang=en&n=24F7211B-1).
Daguet, C., Leli`evre, M., 2019. Identification et protection des corridors naturels de part et d’autre de l’autoroute 10 (Estrie et Montérégie Est) et amélioration de sa perméabilité faunique : premiers résultats. Le Nat. Can. 143 (1), 32–39. https://doi.org/10.7202/1054115ar.
Dumas, K., 2024. Evaluation of wildlife crossing structures and fencing designs to improve effectiveness for herptiles and mammals. Presentation at the Northeastern Transportation & Wildlife Conference (NETWC) in Mystic, CT, 8-11 September 2024. https://www.netwc.org/sessions-2024.html.
Fahrig, L., Rytwinski, T., 2009. Effects of roads on animal abundance: An empirical review and synthesis. Ecol. Soc. 14 (1), 21 http://www.ecologyandsociety.org/vol14/iss1/art21/.
Fahrig, L., Pedlar, J.H., Pope, S.E., Taylor, P.D., Wegner, J.F., 1995. Effect of road traffic on amphibian density. Biological Conservation 73 (3), 177–182. https://doi.org/10.1016/0006-3207(94)00102-V.
Ford, A.T., Clevenger, A.P., Huijser, M.P., Dibb, A., 2011. Planning and prioritization strategies for phased highway mitigation using wildlife-vehicle collision data. Wildl. Biol. 17 (3), 253–265. https://doi.org/10.2981/09-051.
Gouvernement du Québec 2023. Liste des espèces fauniques menacées ou vulnérables. Online: https://www.quebec.ca/agriculture-environnement-et-ressources-naturelles/faune/gestion-faune-habitats-fauniques/especes-fauniques-menacees-vulnerables/liste.
Government of Canada, 2021. Species at Risk Public Registry. Species-Registry. https://www.canada.ca/en/environment-climate-change/services/species-risk-public-registry.html.
Gratton, L., 2014. Protocole d’identification des corridors et passages fauniques - Etude de cas : l’autoroute 10 entre les km 68 et 143. Corridor appalachien. Online: https://www.corridorappalachien.ca/wp-content/uploads/2016/09/protocole_corridors_fauniques_aut10.pdf.
Grilo, C., Neves, T., Bates, J., 420 co-authors, 2025. Global Roadkill Data: A dataset on terrestrial vertebrate mortality caused by collision with vehicles. Scientific Data 12, 505. https://doi.org/10.1038/s41597-024-04207-x.
Grivegnée-Dumoulin, V., Vinet, T., Roy, M., 2022. Plan de connectivité ciblant les aménagements fauniques optimums à prévoir afin d’améliorer la perméabilité faunique et de restaurer la connectivité écologique de part et d’autre de l’autoroute 10 (km 74 à 121). Corridor appalachien, Eastman, Qu´ ebec, p. 127 pp.
Groot Bruinderink, G.W.T.A., Hazebroek, E., 1996. Ungulate traffic collisions in Europe. Conserv. Biol. 10, 1059–1067.
Guinard, É., Julliard, R., Barbraud, C., 2012. Motorways and bird traffic casualties: Carcasses surveys and scavenging bias. Biol. Conserv. 147 (1), 40–51. https://doi.org/10.1016/j.biocon.2012.01.019.
Guinard, E., Prodon, R., Barbraud, C., 2015. Case Study: A robust method to obtain defendable data on wildlife mortality. In: van der Ree, R., Smith, D., & Grilo, C. (Eds.), Handbook of Road Ecology, John Wiley & Sons Ltd, West Sussex, 96–100.
Guinard, E., Billon, L., Bretaud, J.-F., Chevallier, L., Sordello, R., Witté, I., 2023. Comparing the effectiveness of two roadkill survey methods on roads. Transport. Res. Part D: Trans. Environ. 121, 103829. https://doi.org/10.1016/j.trd.2023.103829.
Gunson, K.E., Mountrakis, G., Quackenbush, L.J., 2011. Spatial wildlife-vehicle collision models: A review of current work and its application to transportation mitigation projects. J. Environ. Managem. 92 (4), 1074–1082. https://doi.org/10.1016/j.jenvman.2010.11.027.
Huijser, M.P., Fairbank, E.R., Camel-Means, W., Graham, J., Watson, V., Basting, P., Becker, D., 2016. Effectiveness of short sections of wildlife fencing and crossing structures along highways in reducing wildlife–vehicle collisions and providing safe crossing opportunities for large mammals. Biol. Conserv. 197, 61–68. https://doi.org/10.1016/j.biocon.2016.02.002.
Huijser, M.P., Duffield, J.W., Neher, C., Clevenger, A.P., McGuire, T., 2022. Update and expansion of the WVC mitigation measures and their cost-benefit model. NDOT Research Report No. 701-18-803 TO 1 Part 3. Transportation Pooled Fund Study, TPF-5(358). Nevada Department of Transportation, Carson City, NV. https://doi.org/10.15788/ndot2022.10.
Jaeger, J.A.G., Fahrig, L., 2004. Effects of road fencing on population persistence. Conserv. Biol. 18 (6), 1651–1657. https://doi.org/10.1111/j.1523-
1739.2004.00304.x.
Jaeger, J.A.G., Spanowicz, A.G., Bowman, J., Clevenger, A.P., 2019. Clôtures et passages fauniques pour les petits et moyens mammifères le long de la route 175 au Québec : quelle est leur efficacité ? Le Nat. can. 143 (1), 69–80. https://doi.org/10.7202/1054120ar.
Jakes, A.F., Jones, P.F., Paige, L.C., Seidler, R.G., Huijser, M.P., 2018. A fence runs through it: A call for greater attention to the influence of fences on willdife and ecosystems. Biol. Conserv. 227, 310–318. https://doi.org/10.1016/j.biocon.2018.09.026.
Johnson, C., Jones, D., Matthews, T., Burke, M., 2022a. Advancing avian road ecology research through systematic review. Transport. Res. Part D: Trans. Environ. 109, 103375. https://doi.org/10.1016/.trd.2022.103375.
Johnson, C.D., Matthews, T., Burke, M., Jones, D., 2022b. Planning for fauna-sensitive road design: A review. Front. Envir. Sci. 10, 959918. https://doi.org/10.3389/fenvs.2022.959918.
Johnson, C.D., Matthews, T., Burke, M., Jones, D., 2024. The current attention and traction of fauna-sensitive road design in Australian transport research: A systematic review. Australasian J. Environ. Managem. 31 (3), 296–309. https://doi.org/10.1080/14486563.2024.2377086.
Kang, W., Minor, E.S., Woo, D., Lee, D., Park, C.R., 2016. Forest mammal roadkills as related to habitat connectivity in protected areas. Biodiv. Conserv. 25 (13), 2673–2686. https://doi.org/10.1007/s10531-016-1194-7.
Kavanagh, J., Press, W., 2013. Les Oiseaux du Québec/Quebec Birds: Un guide de poche bilingue sur les esp`eces famili` ere/A Bilingual Folding Pocket Guide to Familiar Species (Fol Lam Ch edition). Waterford Press.
Kociolek, A., Grilo, C., Jacobson, S., 2015. Flight doesn’t solve everything: Mitigation of road impacts on birds. In: van der Ree, R., Smith., D., & Grilo, C. (Eds.), Handbook of Road Ecology, West John Wiley & Sons Ltd, Sussex. 281–289.
Lafrance, M., Alain, É., 2019. Impacts de l’ajout de passages fauniques et du prolongement de clôtures anticervidés sur la sécurité routière de la route 138 à Petite-Rivière-Saint-François. Le Nat. Can. 143 (1), 48–54. https://doi.org/10.7202/1054117ar.
Laliberté, J., St-Laurent, M.H., 2019. Détermination des facteurs spatiotemporels expliquant le risque de collision routière avec des cervidés sur l’autoroute Claude-Béchard (85) au Témiscouata. Le Nat. Can. 143 (1), 40–47. https://doi.org/10.7202/1054116ar.
Langen, T.A., Gunson, K.E., Scheiner, C.A., Boulerice, J.T., 2012. Road mortality in freshwater turtles: Identifying causes of spatial patterns to optimize road planning and mitigation. Biodiv. Conserv. 21 (12), 3017–3034. https://doi.org/10.1007/s10531-012-0352-9.
Nature Conservancy Canada, 2020a. Fisher. https://www.natureconservancy.ca/en/what-we-do/resource-centre/featured-species/mammals/fisher.html.
Nature Conservancy Canada, 2020b. Turtles of Canada. https://www.natureconservancy.ca/en/what-we-do/resource-centre/featured-species/turtles-of-canada.
Neumann, W., Ericsson, G., Dettki, H., Bunnefeld, N., Keuler, N.S., Helmers, D.P., Radeloff, V.C., 2012. Difference in spatiotemporal patterns of wildlife road-crossings and wildlife-vehicle collisions. Biol. Conserv. 145 (1), 70–78. https://doi.org/10.1016/j.biocon.2011.10.011.
Pagany, R., 2020. Wildlife-vehicle collisions – Influencing factors, data collection and research methods. Biol. Conserv. 251, 108758. https://doi.org/10.1016/j.biocon.2020.108758.
Plante, J., Jaeger, J.A.G., Desrochers, A., 2019. How do landscape context and fences influence roadkill locations of small and medium-sized mammals? J. Environ. Managem. 235, 511–520. https://doi.org/10.1016/j.jenvman.2018.10.093.
Prescott, J., Richard, P., 2014. Mammifères du Qu´ ebec et de l’est du Canada, (3rd edition). Quintin Michel.
Robidoux, C., 2019. Passage à tortues de la route 245 à Bolton-Est (Estrie): un bel exemple de partenariat. Le Nat. Can. 143 (1), 85–91. https://doi.org/10.7202/1054122ar.
Roedenbeck, I.A., Fahrig, L., Findlay, C.S., Houlahan, J., Jaeger, J.A.G., Klar, N., Kramer-Schadt, S., van der Grift, E.A., 2007. The Rauischholzhausen agenda for road ecology. Ecol. Soc. 12 (1), 11. https://doi.org/10.5751/ES-02011-120111.
Rytwinski, T., Fahrig, L., 2015. The impacts of roads and traffic on terrestrial animal populations. In: van der Ree, R., Smith, D., & Grilo, C. (Eds.), Handbook of Road Ecology. West Sussex: John Wiley & Sons Ltd. 237–246.
Rytwinski, T., Soanes, K., Jaeger, J.A.G., Fahrig, L., Findlay, C.S., Houlahan, J., van der Ree, R., van der Grift, E.A., 2016. How effective is road mitigation at reducing road-kill? A meta-analysis. PLoS One 11 (11), 1–25. https://doi.org/10.1371/journal.pone.0166941.
Salvant, F., 2017. Identification des zones d’intervention prioritaires pour les mouvements fauniques sur une portion de l’autoroute 10 (Québec, Canada).
Unpublished Master’s thesis. Université de Sherbrooke, Québec, Canada.
Santos, S.M., Carvalho, F., Mira, A., 2011. How long do the dead survive on the road? Carcass persistence probability and implications for road-kill monitoring surveys. PLoS One 6 (9), e25383. https://doi.org/10.1371/journal.pone.0025383.
Santos, R.A.L., Ascens˜ao, F., Ribeiro, M.L., Bager, A., Santos-Reis, M., Aguiar, L.M.S., 2017. Assessing the consistency of hotspot and hot-moment patterns of wildlife road mortality over time. Persp. Ecol. Conserv. 15 (1), 56–60. https://doi.org/10.1016/j.pecon.2017.03.003.
Slater, F.M., 2002. An assessment of wildlife road casualties—The potential discrepancy between numbers counted and numbers killed. Web Ecology 3 (1), 33. https://doi.org/10.5194/we-3-33-2002.
Société d’histoire naturelle de la vallée du Saint-Laurent (SHNVSL), 2015. Guide de conservation des amphibiens, des reptiles et de leurs habitats en milieu agricole. Sainte-Anne-de-Bellevue, Québec. https://oaq.qc.ca/wp-content/uploads/2016/05/SHNVSL_Guide-amphibiens-reptiles-milieu-agricole_lowres_v2.pdf.
Spanowicz, A.G., Teixeira, F.Z., Jaeger, J.A.G., 2020. An adaptive plan for prioritizing road sections for fencing to reduce animal mortality. Conserv. Biol. 34 (5), 1210–1220. https://doi.org/10.1111/cobi.13502.
Steen, D.A., Aresco, M.J., Beilke, S.G., Compton, B.W., Condon, E.P., Kenneth Dodd Jr, H., Forrester, H., Gibbons, J.W., Greene, J.L., Johnson, G., Langen, T.A., Oldham, M.J., Oxier, D.N., Saumure, R.A., Schueler, F.W., Sleeman, J.M., Smith, L.L., Tucker, J.K., Gibbs, J.P., 2006. Relative vulnerability of female turtles to road mortality. Animal Conserv. 9 (3), 269–273. https://doi.org/10.1111/j.1469-1795.2006.00032.x.
St-Pierre, D., Nappi, A., de Bellefeuille, S., Lévesque Aubé, A.A., Martin, S., 2019. La connectivité au-delà des frontières : Résolution 40-3 concernant la connectivité écologique, l’adaptation aux changements climatiques et la conservation de la biodiversité. Le Nat. Can. 143 (1), 8–11. https://doi.org/10.7202/1054111ar.
Teixeira, F.Z., Coelho, I.P., Esperandio, I.B., Oliveira, N., 2013a. Are road-kill hotspots coincident among different vertebrate groups? Oecol. Austral. 17 (17), 36–47. https://doi.org/10.4257/oeco.2013.1701.04.
Teixeira, F.Z., Coelho, A.V.P., Esperandio, I.B., Kindel, A., 2013b. Vertebrate road mortality estimates: Effects of samping methods and carcass removal. Biol. Conserv. 157, 317–323. https://doi.org/10.1016/j.biocon.2012.09.006.
Teixeira, F.Z., Kindel, A., Hartz, S.M., Mitchell, S., Fahrig, L., 2017. When roadkill hotspots do not indicate the best sites for road-kill mitigation. J. Appl. Ecol. 54 (5), 1544–1551. https://doi.org/10.1111/1365-2664.12870.
Valerio, F., Mira, A., Santos, S.M., Barbosa, A.M., Carvalho, F., 2019. Accounting for connectivity uncertainties in predicting roadkills: A comparative approach between path selection functions and habitat suitability models. Environ. Managem. 64 (3), 329–343. https://doi.org/10.1007/s00267-019-01191-6.
Vallée, P.-M., Bélanger, J., Fortin, J., 2019. Conception et construction des aménagements relatifs aux cerfs de Virginie le long de l’autoroute Robert-Cliche (A-73) au Québec. Le Nat. Can. 143 (1), 55–61. https://doi.org/10.7202/1054118ar.
van der Ree, R., Smith., D. Grilo, C. (Eds.) 2015. Handbook of Road Ecology. John Wiley & Sons Ltd, West Sussex.
Wang, Y., Guan, L., Chen, J., Kong, Y., 2018. Influences on mammals frequency of use of small bridges and culverts along the Qinghai-Tibet railway, China. Ecological Research 33, 879–887. https://doi.org/10.1007/s11284-018-1578-0.
Ward, M.R., Stallknecht, D.E., Willis, J., Conroy, M.J., Davidson, W.R., 2006. Wild bird mortality and West Nile virus surveillance: Biases associated with detection, reporting, and carcass persistence. Journal of Wildlife Diseases 42 (1), 92–106, 16699152.
Warnock-Juteau, K., Bolduc, V., LoScerbo, D., Anderson, M., Daguet, C., Jaeger, J.A.G., 2022. Co-use of existing crossing structures along roads by wildlife and humans: Wishful thinking? Nature Conserv. 47, 235–270. https://natureconservation.pensoft.net/article/73060/.
Wilansky, J., Jaeger, J.A.G., 2024. Predicting the effectiveness of wildlife fencing along roads using an individual-based model: How do fence-following distances influence the fence-end effect? Ecol. Model. 495, 110784. https://doi.org/10.1016/j.ecolmodel.2024.110784.
Wilkins, D.C., Kockelman, K.M., Jiang, N., 2019. Animal-vehicle collisions in Texas: How to protect travelers and animals on roadways. Acc. Analysis Prev. 131,157–170. https://doi.org/10.1016/j.aap.2019.05.030.
Williams, S.T., Collinson, W., Patterson-Abrolat, C., Marneweck, D.G., Swanepoel, L.H., 2019. Using road patrol data to identify factors associated with carnivoreroadkill counts. PeerJ 7, e6650. https://doi.org/10.7717/peerj.6650.
Wintle, B.A., McCarthy, M.A., Parris, K.M., Burgman, M.A., 2004. Precision and bias of methods for estimating point survey detection probabilities. Ecol. Applic. 14(3), 703–712. https://doi.org/10.1890/02-5166.
Woodroffe, R., Hedges, S., Durant, S.M., 2014. To fence or not to fence. Science 344, 46–48. https://doi.org/10.1126/science.1246251.
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