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Light regulation of the RNA-binding activity of 46 kDa protein in chloroplast of Chlamydomonas reinhardtii

Title:

Light regulation of the RNA-binding activity of 46 kDa protein in chloroplast of Chlamydomonas reinhardtii

Wang, Shengwu (2001) Light regulation of the RNA-binding activity of 46 kDa protein in chloroplast of Chlamydomonas reinhardtii. Masters thesis, Concordia University.

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Abstract

In this thesis project, I undertook a study of the light regulation of a 46 kDa RNA binding protein associated with chloroplast membranes in C. reinhardtii . The optimal conditions for binding of this protein were characterized. Initial purification steps using (NH 4 ) 2 SO 4 precipitation and affinity chromatography revealed that this protein is present at extremely low abundance. This activity is low in the dark and increases drastically within 1-10 minute following a shift to light. This activation does not require protein synthesis in either the chloroplast or the cytosol. Finally activation is abolished by a proton ionophore and an inhibitor of the cytochrome b 6 /f complex, and partially reduced by an inhibitor of photosystem II. With a previous finding that ADP inhibits the RNA-binding activity of the protein in vitro , these results support a role of ADP in repression of this binding activity.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Thesis (Masters)
Authors:Wang, Shengwu
Pagination:xiv, 98 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:Theses (M.Sc.)
Program:Biology
Date:2001
Thesis Supervisor(s):Zerges, William
ID Code:1442
Deposited By:Concordia University Libraries
Deposited On:27 Aug 2009 13:19
Last Modified:08 Dec 2010 10:20
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