Wang, Shengwu (2001) Light regulation of the RNA-binding activity of 46 kDa protein in chloroplast of Chlamydomonas reinhardtii. Masters thesis, Concordia University.
In this thesis project, I undertook a study of the light regulation of a 46 kDa RNA binding protein associated with chloroplast membranes in C. reinhardtii . The optimal conditions for binding of this protein were characterized. Initial purification steps using (NH 4 ) 2 SO 4 precipitation and affinity chromatography revealed that this protein is present at extremely low abundance. This activity is low in the dark and increases drastically within 1-10 minute following a shift to light. This activation does not require protein synthesis in either the chloroplast or the cytosol. Finally activation is abolished by a proton ionophore and an inhibitor of the cytochrome b 6 /f complex, and partially reduced by an inhibitor of photosystem II. With a previous finding that ADP inhibits the RNA-binding activity of the protein in vitro , these results support a role of ADP in repression of this binding activity.
|Divisions:||Concordia University > Faculty of Arts and Science > Biology|
|Item Type:||Thesis (Masters)|
|Pagination:||xiv, 98 leaves : ill. ; 29 cm.|
|Degree Name:||Theses (M.Sc.)|
|Thesis Supervisor(s):||Zerges, William|
|Deposited By:||Concordia University Libraries|
|Deposited On:||27 Aug 2009 17:19|
|Last Modified:||08 Dec 2010 15:20|
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