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Single label fluorescent derivatization of peptides

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Single label fluorescent derivatization of peptides

Little, Michael J (1997) Single label fluorescent derivatization of peptides. Masters thesis, Concordia University.

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Abstract

A one step method for the attachment of a single fluorescent label to peptides through their N-terminus is described. The method employs fluorescein-5-isothiocyanate as the label of choice and a lower than normal derivatization buffer pH that discriminates against $\varepsilon$-amino derivatization in favor of $\alpha$-amino derivatization provided that this group may act as an efficient nucleophile. A kinetic study was performed in order to identify the optimal derivatization buffer pH for $\alpha$-amino group selectivity, using $\rm N\alpha$-t-BOC-L-lysine and $\rm N\varepsilon$-t-BOC-L-lysine as model compounds for derivatization through $\varepsilon$-amino and $\alpha$-amino groups, respectively. The optimal pH was found to be 8.5. Six small peptides, Arg-Lys, Lys-Trp-Lys, Lys-Lys, Lys-Lys-Lys, Lys-Lys-Lys-Lys and Arg-Pro-Lys-Pro, were derivatized at pH 8.5 in an effort to corroborate the results of the kinetic study. It was found that this method of single label derivatization via buffer pH control was successful for peptides with fewer than four lysines residues.

Divisions:Concordia University > Faculty of Arts and Science > Chemistry and Biochemistry
Item Type:Thesis (Masters)
Authors:Little, Michael J
Pagination:xi, 90 leaves : ill. ; 29 cm.
Institution:Concordia University
Degree Name:Theses (M.Sc.)
Program:Dept. of Chemistry and Biochemistry
Date:1997
Thesis Supervisor(s):Bonk, Peter
ID Code:380
Deposited By:Concordia University Libraries
Deposited On:27 Aug 2009 13:11
Last Modified:08 Dec 2010 10:14
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