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Microsatellite analysis reveals genetically distinct populations of red pine (Pinus resinosa, Pinaceae)

Title:

Microsatellite analysis reveals genetically distinct populations of red pine (Pinus resinosa, Pinaceae)

Boys, Jacquelyn and Cherry, Marilyn and Dayanandan, Selvadurai (2005) Microsatellite analysis reveals genetically distinct populations of red pine (Pinus resinosa, Pinaceae). American Journal of Botany, 92 (5). pp. 833-841. ISSN 0002-9122

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Official URL: http://www.amjbot.org/cgi/reprint/92/5/833

Abstract

Red pine (Pinus resinosa Ait.) is an ecologically and economically important forest tree species of northeastern North America and is considered one of the most genetically depauperate conifer species in the region. We have isolated and characterized 13 nuclear microsatellite loci by screening a partial genomic library with di-, tri-, and tetranucleotide repeat oligonucleotide probes. In an analysis of over 500 individuals representing 17 red pine populations from Manitoba through Newfoundland, five polymorphic microsatellite loci with an average of nine alleles per locus were identified. The mean expected and observed heterozygosity values were 0.508 and 0.185, respectively. Significant departures from Hardy-Weinberg equilibrium with excess homozygosity indicating high levels of inbreeding were evident in all populations studied. The population differentiation was high with 28–35% of genetic variation partitioned among populations. The genetic distance analysis showed that three northeastern (two Newfoundland and one New Brunswick) populations are genetically distinct from the remaining populations. The coalescence-based analysis suggests that "northeastern" and "main" populations likely became isolated during the most recent Pleistocene glacial period, and severe population bottlenecks may have led to the evolution of a highly selfing mating system in red pine.

Divisions:Concordia University > Faculty of Arts and Science > Biology
Item Type:Article
Refereed:Yes
Authors:Boys, Jacquelyn and Cherry, Marilyn and Dayanandan, Selvadurai
Journal or Publication:American Journal of Botany
Date:May 2005
Funders:
  • Concordia University
  • Natural Sciences and Engineering Research Council of Canada
  • Fonds Québécois de la Recherche sur la Nature et les Technologies
  • Canada Foundation for Innovation
Keywords:genetic bottleneck; genetic diversity; microsatellites; Pinus; Pleistocene refugia; population genetics; Postglacial colonization
ID Code:6702
Deposited By:DANIELLE DENNIE
Deposited On:08 Jun 2010 17:16
Last Modified:08 Dec 2010 18:18
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